kofi

Nutrition. 2008 Nov-Dec;24(11-12):1189-98. Epub 2008 Aug 23.
*
Short-chain fatty acids and trichostatin A alter tight junction
permeability in human umbilical vein endothelial cells.
Miyoshi M, Usami M, Ohata A.
Division of Nutrition and Metabolism, Kobe University Graduate School of
Health Sciences, Kobe, Japan.

OBJECTIVE: The short-chain fatty acids (SCFAs), butyrate, propionate,
and acetate, produced by bacterial fermentation of dietary fiber, can
modulate the transcription of certain genes by inhibiting histone
deacetylase in colonocytes and several other cell types in vitro. We
previously reported that butyrate decreases tight junction permeability
by activating lipoxygenase (LOX) in intestinal monolayer cells. In the
present study, we evaluated the effects of SCFAs on tight junction
permeability in an endothelial cell culture and their possible
mechanisms of action via histone deacetylase inhibitor activity. We also
investigated the factors modulating tight junction permeability.
METHODS: The effects of butyrate, propionate, and acetate on tight
junction permeability in human umbilical vein endothelial cells were
examined using Transwell chamber cultures. The contributions of
inducible nitric oxide synthase (NOS), endothelial NOS, estrogen
receptor, cyclo-oxygenase, and LOX to SCFAs' effects were also
evaluated. The effects of SCFAs were compared with those of trichostatin
A, a typical histone deacetylase inhibitor. RESULTS: Low concentrations
of butyrate and propionate decreased paracellular permeability without
inducing cell damage. However, acetate decreased paracellular
permeability in a concentration-dependent manner. An estrogen receptor
antagonist attenuated the effects of SCFAs on tight junction
permeability. The influences of inducible NOS, cyclo-oxygenase, and LOX
inhibitors and the expressions of cyclo-oxygenase and LOX mRNAs were
different for each SCFA. Trichostatin A slightly decreased paracellular
permeability when used at lower concentrations, but higher
concentrations of trichostatin A increased permeability. CONCLUSION: The
SCFAs play an important role in the assembly of tight junctions in
normal vascular endothelial cells.

Publication Types:
* Research Support, Non-U.S. Gov't

PMID: 18723323

Kofi, what would you advice in order to decrease intestinal
permebaility?

Butyrate (if yes in which form? Hydroxymethyl?) and propionate?

kofi

I suspect individual response and dosage are highly variable, so you may
need to experiment. Butyrate has a habit of reawakening latent
herpesvirus infections (CMV, EBV, etc.). If you can tolerate it, there
are a number of forms in which you can take it. Carnitine should
enhance its absorption. I'd imagine vitamin D3 is also particularly
helpful in conjunction with butyrate.

Ye, that's the problem, I don't tolerate Butyrate that well. My
vitamin 25(OH) Vitmain D3 levels are normal but I try to raise them a
little more. How about propionate? It's time to make the Zonulin
inhibitor (AT-1001) available.

pramesh rutaji

Or perhapses I might make one more susceptible to infection?

BHT (butylated hydroxytoluene) may fight and/or help prevent said
infections in the first place?

--

Pramesh Rutaji

p297tongue6221@newsguy.com - remove tongue to reply

kofi

I think there is a carnitine proprionate available but proprionate isn't
studied as well as butyrate.

pramesh rutaji

Or perhapses it might make one more susceptible to infection?

BHT (butylated hydroxytoluene) may fight and/or help prevent said
infections in the first place?

--

Pramesh Rutaji

p297tongue6221@newsguy.com - remove tongue to reply