Roger 2009-04-07 17:14:21
That’s pancreatic, bladder, and prostate cancer that is inhibited by
lipoxygenase inhibitors (see the previous two abstracts I just posted).
Might work for most if not all cancers.
J Urol. 2003 Nov;170(5):1994-9.
Expression of lipoxygenase in human bladder carcinoma and growth inhibition
by its inhibitors.
Yoshimura R, Matsuyama M, Tsuchida K, Kawahito Y, Sano H, Nakatani T.
Department of Urology, Osaka City University Medical School, 1-4-3
Asahi-machi, Abenoku, Osaka 545-8585, Japan
PURPOSE: The metabolism of arachidonic acid by the cyclooxygenase or
lipoxygenase pathway generates eicosanoids, which have been implicated in
the pathogenesis of various human diseases, including cancer. They are now
believed to have important roles in tumor promotion, progression and
metastasis. The involvement of lipoxygenase expression and function in tumor
growth and metastasis has been reported in human tumor cell lines.
MATERIALS AND METHODS: The expression of 5 and 12-lipoxygenase in patients
with bladder tumor and chronic cystitis, and in normal bladder tissues was
examined. We also examined the effects of their inhibitors on cell
proliferation in a bladder cancer cell line. The expression of 5 and
12-lipoxygenase protein was detected by immunohistochemistry. The effects of
lipoxygenase inhibitors on bladder cancer cell growth were examined by MTT
(3-[4,5-dimethylthiazol-2-thiazolyl]-2,5-diphenyltetrazolium bromide) assay,
while Hoechst (Sigma Chemical Co., St. Louis, Missouri) staining was used to
determine whether lipoxygenase inhibitors induce apoptosis.
RESULTS: While slight 5 and 12-lipoxygenase expression was detected in
chronic cystitis and normal bladder tissues, marked 5 and 12-lipoxygenase
expression was detected in bladder cancer tissues. Lipoxygenase inhibitors
caused marked inhibition of bladder cancer cells in a concentration and time
dependent manner. Cells treated with lipoxygenase inhibitors showed
chromatin condensation, cellular shrinkage, small membrane bound bodies
(apoptotic bodies) and cytoplasmic condensation.
CONCLUSIONS: Lipoxygenase is induced in bladder cancer. Results suggest that
lipoxygenase inhibitors may mediate potent antiproliferative effects against
bladder cancer cells. Thus, lipoxygenase may become a new target in the
treatment of bladder tumors.