Login failed for user '(null)'. Reason: Not associated VS ASP.NEt

ASP.NET vs SQL Server¸

I have an ASP.NET Application
In IIS I checked the Integrated Windows Authentication
option

In Web.Config the connection string include Integrated
Security = SSPI

When I start then Web Application I have this messge
Login failed for user '(null)'. Reason: Not associated
with a trusted SQL Server connection.
Could you help me

PS My account have the permission to connect to SQl Server

the security model is base on role security
I have many users

Please refer to
http://support.microsoft.com/default.aspx?scid=kb;en-us;316989

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I use Impersonate = true

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2 22nd January 00:41 gandhinath swaminathan\(ms\) External User Posts: 1	Login failed for user '(null)'. Reason: Not associated VS ASP.NEt Please refer to http://support.microsoft.com/default.aspx?scid=kb;en-us;316989 -- -- This posting is provided "AS IS" with no warranties, and confers no rights. Use of included script samples are subject to the terms specified at http://www.microsoft.com/info/copyright.htm.

3 22nd January 00:41 rl30 External User Posts: 1	Login failed for user '(null)'. Reason: Not associated VS ASP.NEt I use Impersonate = true